Fig. 6. Expression patterns of different signaling pathway proteins after PKM2 knockdown by siRNAs (100 nM for 72 h). (A) The effect of PKM2 knockdown on anticancer signaling pathways in DU145 cells. The cells were transfected with the indicated siRNAs, and western blotting was performed to determine the expression levels of the different signaling pathway proteins. Representative blots are shown. (B) The intensities of the bands were measured and depicted in the bar graph by the ratio of GAPDH. (C) The ratio of p-Akt/Akt, p-mTOR/mTOR, p-p70S6K/ p70S6K and p-GSK3β/ GSK3β were measured and depicted in the bar graph. One-way ANOVA was used to compare the means of different groups. Differences between the means were considered to be significant at P<0.05 by using Tukey multiple comparison tests; ^*P<0.05, **P<0.01, and ***P<0.001 compared with the normal control cells.